ERNDIM

Training and Education

Educational Documents


General Oligosaccharide kit White cell cystine assay

General

Amino Acid Analysis Recommendations
1-May-2002
S. Holtrop, N.G.GM. Abeling and A.H. van Gennip Dept. of Clinical Chemistry, AMC,Amsterdam, The Netherlands

Recommendations to improve the quality of diagnostic quantitative analysis of amino acids in plasma and urine using cation-exchange liquid chromatography with post column ninhydrin reaction and detection

Control of Accuracy and Precision
1-May-2002
C. Bachmann Laboratoire Central de Chimie Clinique Centre Hospitalier Universitaire Vaudois CH 1011 Lausanne, Switzerland

The control of analytical accuracy and day to day precision helps for the follow-up of patients and is essential when using biochemical data from the literature.

Theoretical aspect of QC in IEM and Method validation
1-May-2002
Dr. P. Divry, Lyon; Prof. B. François, Chairman, Diepenbeek; Dr. A.C. Sewell, Frankfurt; Dr. L. Spaapen, Maastricht. C. Vandervorst, Diepenbeek.

Quality control in inherited disorders of metabolism (IDM) laboratories. ERNDIM schemes and theoretical aspects.

Mixed Leucocyte Preparation
1-May-2001
Dr. Neil Dalton MA PhD Children Nationwide Senior Lecturer in Paediatric Biochemistry Guy's, King's College, & St. Thomas' Hospitals Medical School Department of Paediatrics Guy's Hospital

The preparation method has been in use at Guy's Hospital, London for the last 12 years and was originally established under the guidance of Professor J Schneider, UCSD

Polymorphonuclear Leucocyte Preparation
1-May-2001
Dr. Bernadette Chadefaux-Vekemans Laboratoire de Biochimie Médicale

Polymorphonuclear leucocytes should be the biological material of choice for measurement of cystine because cystine in cystinotic leucocytes is located primarily in phagocytic blood cells (polynuclear and monocytes) rather than in lymphocytes.

Purine and Pyrimidine Analysis
1-May-2001
Jörgen Bierau, Ph.D., clinical biochemical geneticist Laboratory of Biochemical Genetics Department of Clinical Genetics Maastricht University Medical Centre

ERNDIM ADVISORY DOCUMENT ON THE QUANTITATIVE ANALYSIS OF PURINES AND PYRIMIDINES.

Role of EQA in special assays for IEM
1-May-2001
M.Duran – Academic Medical Center Amsterdam

THE ERNDIM SPECIAL ASSAYS SCHEME
PROFITS, PITFALLS, DRAWBACKS AND EXPECTATIONS

Biomed 2 Recommendations
7-Mar-2000
J. Bonham, A. van Gennip, B. François, C. Bachmann, P. Divry, B. Fowler

Biomed 2 - Subproject B
Report summarising achievements and final recommendations

Oligosaccharide kit (Top)

Oligosaccharide Kit - TLC separation picture September 2016
11-Aug-2017
Dr G.J.G. Ruijter

TLC separation of the Oligosaccharide kit available for purchase on a not for profit basis from SKML (http://cms.erndimqa.nl/Educational-Panels.aspx)

Protocol for qualitative TLC Oligosaccharide analysis
3-Apr-2013
G. Ruijter

White cell cystine assay (Top)

White cell cystine assay Calculation of final results using an Excel spreadsheet (Doc 4/4)
19-Jan-2017
Mick Henderson & Daniel Herrera

This document is part of a 4 document series on the ERNDIM website, which give details of methods for Cysteine analysis in White Blood Cells. These are the methods used in the laboratory of the Scientific Advisor for the ERNDIM Cystine in WBC EQA scheme and any queries should be directed to mick.henderson@nhs.net or daniel.herrera@nhs.net.

White cell cystine Assay Stage 1 Isolation of a white cell pellet (Doc 1/4)
19-Jan-2017
Mick Henderson & Daniel Herrera

This document is part of a 4 document series on the ERNDIM website, which give details of methods for Cysteine analysis in White Blood Cells. These are the methods used in the laboratory of the Scientific Advisor for the ERNDIM Cystine in WBC EQA scheme and any queries should be directed to mick.henderson@nhs.net or daniel.herrera@nhs.net.

White cell cystine assay Stage 2 Cystine analysis by Tandem MS (Doc 2/4)
19-Jan-2017
Mick Henderson & Daniel Herrera

This document is part of a 4 document series on the ERNDIM website, which give details of methods for Cysteine analysis in White Blood Cells. These are the methods used in the laboratory of the Scientific Advisor for the ERNDIM Cystine in WBC EQA scheme and any queries should be directed to mick.henderson@nhs.net or daniel.herrera@nhs.net.

White cell cystine assay White Cell Protein Analysis (Doc 3/4)
19-Jan-2017
Mick Henderson & Daniel Herrera

This document is part of a 4 document series on the ERNDIM website, which give details of methods for Cysteine analysis in White Blood Cells. These are the methods used in the laboratory of the Scientific Advisor for the ERNDIM Cystine in WBC EQA scheme and any queries should be directed to mick.henderson@nhs.net or daniel.herrera@nhs.net.